Active Recombinant Human SHH protein(Cys24Ile-Val-Ile)

• Cat.No.: SHH-2603H
• Product Overview: Recombinant Human SHH protein(Cys24-Gly197(Cys24Ile-Val-Ile)) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 24-197 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 300mM NaCl, pH 7.4.
• Bio-activity: Measured by its ability to inhibit p53 expression in C3H10T1/2 mouse embryonic fibroblast cells. 1.25-2.5 μg/mL of Recombinant Human Sonic hedgehog can effectively decrease p53 expression.
• Molecular Mass: 19.78 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE; ≥95 % as determined by HPLC.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: SHH sonic hedgehog [ Homo sapiens ]
• Official Symbol: SHH
• Synonyms: SHH; sonic hedgehog; HLP3, HPE3, sonic hedgehog (Drosophila) homolog , sonic hedgehog homolog (Drosophila); sonic hedgehog protein; HHG1; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; HLP3; HPE3
• Gene ID: 6469
• mRNA Refseq: NM_000193
• Protein Refseq: NP_000184
• MIM: 600725
• UniProt ID: Q15465