Recombinant Human SHPRH protein, His-tagged

• Cat.No.: SHPRH-2666H
• Product Overview: Recombinant Human SHPRH protein(1334-1683 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1334-1683 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: EYKLLHEYWMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAFATSQLQKKLGQLLYLTNLEKSQDKTSGGVNPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKANQEEDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKRDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAERSHTNSSAKHSEASVLTVADLADLFTKETEELE
• Purity: 80%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 12 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: SHPRH SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: SHPRH
• Synonyms: SHPRH; SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase; SNF2 histone linker PHD RING helicase; E3 ubiquitin-protein ligase SHPRH; bA545I5.2; FLJ90837; KIAA2023; 2610103K11Rik; SNF2, histone-linker, PHD and RING finger domain-containing helicase; FLJ27258; FLJ37625; FLJ45012; MGC134886
• Gene ID: 257218
• mRNA Refseq: NM_001042683
• Protein Refseq: NP_001036148
• MIM: 608048
• UniProt ID: Q149N8