Recombinant Human SI protein, His & S-tagged

• Cat.No.: SI-7918H
• Product Overview: Recombinant Human SI aa. (Asn1717~Ser1827 (Accession # P14410)) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&S
• Protein Length: Asn1717~Ser1827
• Description: This gene encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 18.3kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.2.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: SI sucrase-isomaltase [ Homo sapiens (human) ]
• Official Symbol: SI
• Synonyms: SI; sucrase-isomaltase; sucrase-isomaltase, intestinal; alpha-glucosidase; oligosaccharide alpha-1,6-glucosidase
• Gene ID: 6476
• mRNA Refseq: NM_001041.3
• Protein Refseq: NP_001032.2
• UniProt ID: P14410