Recombinant Human SIGLEC10 protein, His-tagged

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Recombinant Human SIGLEC10 protein, His-tagged

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Cat.No. : SIGLEC10-059H
Product Overview : Human Siglec-2/CD22 is expressed from mammalian with a His tag and Avi Tag at the C-terminal. It contains Asp20-Arg687.
Description : Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Source : HEK293
Species : Human
Tag : His
Form : Supplied as 0.22um filtered solution in 20mM PB, 150mM Nal, 25mM Arginine(pH 7.4).
Molecular Mass : The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 100- 115KDa based on Bis-Tris PAGE result.
Protein length : It contains Asp20-Arg687.
Endotoxin : Less than 0.05EU per ug by the LAL method.
Purity : > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Storage : The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Tris-Bis PAGE :

HPLC data :

ELISA data :

Gene Name : SIGLEC10 sialic acid binding Ig-like lectin 10 [ Homo sapiens ]
Official Symbol : SIGLEC10
Synonyms : siglec 10; siglec-10; siglec10; Sialic acid-binding Ig-like lectin 10; Siglec-like protein 2; SLG2
Gene ID : 89790
mRNA Refseq : NM_001171156
Protein Refseq : NP_001164627
MIM : 606091
UniProt ID : Q96LC7

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