Recombinant Human SIGLEC10 protein, Mouse IgG2a Fc-tagged, low endotoxin
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|Product Overview :||Recombinant Human SIGLEC10 protein (Met17-Thr546)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.|
|Tag :||Mouse IgG2a Fc|
|Form :||Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 85.6 kDa. The protein migrates as 90-120 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||530|
|Endotoxin :||Less than 0.1 EU per ug by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of SIGLEC10-331H :||
Human Siglec-10, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of SIGLEC10-331H :||
Immobilized Human Siglec-10, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Anti-Human Siglec-10 MAb, human IgG with a linear range of 0.6-20 ng/mL (QC tested).
|Gene Name :||SIGLEC10|
|Official Symbol :||SIGLEC10|
|Synonyms :||SIGLEC10; sialic acid binding Ig-like lectin 10; sialic acid-binding Ig-like lectin 10; MGC126774; PRO940; sialic acid binding Ig like lectin 10 Ig like lectin 7; SIGLEC 10; siglec like gene 2; SLG2; siglec-like gene 2; siglec-like protein 2; sialic acid binding Ig-like lectin 10 Ig-like lectin 7; SIGLEC-10|
|Gene ID :||89790|
|mRNA Refseq :||NM_001171156|
|Protein Refseq :||NP_001164627|
|UniProt ID :||Q96LC7|
◆ Recombinant Protein
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