"SIGLEC15" Related Products


Recombinant Human SIGLEC15 protein, Fc-tagged

Cat.No.: SIGLEC15-256H
Product Overview: Recombinant Human SIGLEC15(Phe20-Thr263) fused Fc region of Human IgG1 at C-terminal was expressed in CHO.
Description: Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10. It is expressed on osteoclasts, macrophages, and dendritic cells and binds to the sialyl-Tn antigen. This interaction induces the production of TGF-beta by tumor-associated macrophages. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
Source: CHO
Species: Human
Tag : Fc
Predicted N Terminal: Phe20
Form: Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED50 of 150-900 ng/mL.
Molecular Mass: Predicted Molecular Mass: 53 kDa
SDS-PAGE: 53-64 kDa, reducing conditions
Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 200 μg/mL in water.
Gene Name: SIGLEC15 sialic acid binding Ig like lectin 15 [ Homo sapiens ]
Official Symbol: SIGLEC15
Synonyms: CD33L3; HsT1361; SIGLEC-15; CD33 antigen-like 3; CD33 molecule-like 3
Gene ID: 284266
mRNA Refseq: NM_213602
Protein Refseq: NP_998767
UniProt ID: Q6ZMC9

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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