Active Recombinant Human SIGLEC15 protein, His-Avi-tagged, Low Endotoxin

• Cat.No.: SIGLEC15-170H
• Product Overview: Recombinant Human SIGLEC15 protein(Q6ZMC9)(20-263 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 20-263 aa
• Form: Lyophilized from 0.22μm filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Siglec-15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.