Recombinant Human SIGLEC15 protein, His-tagged SIGLEC15-1137H

Recombinant Human SIGLEC15 protein, His-tagged

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Recombinant Human SIGLEC15 protein, His-tagged

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Cat.No. : SIGLEC15-1137H
Product Overview : Recombinant SIGLEC15 protein (Phe20-Thr263), fused to His-tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass : 28.4 kDa
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
SDS-PAGE of SIGLEC15-1137H :

Human Siglec-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of SIGLEC15-1137H :

Immobilized Human Siglec-15, His Tag at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.3-2 ng/mL (QC tested).

Immobilized Human Siglec-15, His Tag at 10 μg/mL (100 μL/well) on an Nickel Coated plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-5 μg/mL (Routinely tested).

Bioactivity-BLI of SIGLEC15-1137H :

Loaded Mouse Human chimeric MAb (5G12, Human IgG1) on AHC Biosensor, can bind Human Siglec-15, His Tag with an affinity constant of 0.52 nM as determined in BLI assay (Routinely tested).

Gene Name : SIGLEC15
Official Symbol : SIGLEC15
Synonyms : CD33 antigen-like 3; SIGLEC-15; CD33L3; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15
Gene ID : 284266
mRNA Refseq : NM_213602.3
Protein Refseq : NP_998767.1
MIM : 618105
UniProt ID : Q6ZMC9

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