Recombinant Human SIGLEC15 protein, His-tagged
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|Product Overview :||Recombinant SIGLEC15 protein (Phe20-Thr263), fused to His-tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Molecular Mass :||28.4 kDa|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|SDS-PAGE of SIGLEC15-1137H :||
Human Siglec-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of SIGLEC15-1137H :||
Immobilized Human Siglec-15, His Tag at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.3-2 ng/mL (QC tested).
Immobilized Human Siglec-15, His Tag at 10 μg/mL (100 μL/well) on an Nickel Coated plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-5 μg/mL (Routinely tested).
|Bioactivity-BLI of SIGLEC15-1137H :||
Loaded Mouse Human chimeric MAb (5G12, Human IgG1) on AHC Biosensor, can bind Human Siglec-15, His Tag with an affinity constant of 0.52 nM as determined in BLI assay (Routinely tested).
|Gene Name :||SIGLEC15|
|Official Symbol :||SIGLEC15|
|Synonyms :||CD33 antigen-like 3; SIGLEC-15; CD33L3; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15|
|Gene ID :||284266|
|mRNA Refseq :||NM_213602.3|
|Protein Refseq :||NP_998767.1|
|UniProt ID :||Q6ZMC9|
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