Active Recombinant Human SIRT3 protein, His-tagged

• Cat.No.: SIRT3-10H
• Product Overview: Active Recombinant Human SIRT3 protein(Q9NTG7)(126-399 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 126-399 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Sirtuin 3 (SIRT3), the NAD-dependent deacetylaseis is member of the mammalian sirtuin family of proteins. In human, sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. It also can regulate epigenetic genesilencing and suppress recombination of rDNA in yeast. SIRT3 expression in white and brown adipose tissue. Besides, Isocitrate Dehydrogenase2, mitochondrial (IDH2) has been identified as an interact or of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant humant SIRT3 and recombinant human IDH2. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IDH2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SIRT3 pAb, thenaspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add50µLstop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and IDH2 was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 31.7kDa
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SIRT3 sirtuin 3 [ Homo sapiens ]
• Official Symbol: SIRT3
• Synonyms: SIRT3; sirtuin 3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; SIR2L3; sir2-like 3; sirtuin type 3; SIR2-like protein 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• Gene ID: 23410
• mRNA Refseq: NM_001017524
• Protein Refseq: NP_001017524
• MIM: 604481
• UniProt ID: Q9NTG7