Recombinant Human SIRT7 protein, His-tagged

• Cat.No.: SIRT7-3230H
• Product Overview: Recombinant Human SIRT7 protein(199-400 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 199-400 aa
• AA Sequence: VPNREYVRVFDVTERTALHRHQTGR TCHKCGTQLRDTIVHFGERGTLGQP LNWEAATEAASRADTILCLGSSLKV LKKYPRLWCMTKPPSRRPKLYIVNL QWTPKDDWAALKLHGKCDDVMRLLM AELGLEIPAYSRWQDPIFSLATPLR AGEEGSHSRKSLCRSREEAPPGDRG APLSSAPILGGWFGRGCTKRTKRKK VT
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: SIRT7 sirtuin 7 [ Homo sapiens ]
• Official Symbol: SIRT7
• Synonyms: SIRT7; sirtuin 7; sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7; NAD-dependent deacetylase sirtuin-7; sirtuin type 7; SIR2-like protein 7; sir2-related protein type 7; silent mating type information regulation 2, S.cerevisiae, homolog 7; SIR2L7; MGC126840; MGC126842
• Gene ID: 51547
• mRNA Refseq: NM_016538
• Protein Refseq: NP_057622
• MIM: 606212
• UniProt ID: Q9NRC8