Recombinant Human SLAMF7 cell lysate

Cat.No. : SLAMF7-2378HCL
Product Overview : Human SLAMF7 / CRACC / CD319 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Tag : Non
Gene Name : SLAMF7 SLAM family member 7 [ Homo sapiens ]
Official Symbol : SLAMF7
Synonyms : SLAMF7; SLAM family member 7; 19A; CD319; CRACC; CS1; protein 19A; CD2 subset 1; 19A24 protein; membrane protein FOAP-12; CD2-like receptor activating cytotoxic cells; CD2-like receptor-activating cytotoxic cells; novel LY9 (lymphocyte antigen 9) like protein;
Gene ID : 57823
mRNA Refseq : NM_021181
Protein Refseq : NP_067004
MIM : 606625
UniProt ID : Q9NQ25
Chromosome Location : 1q23.1-q24.1
Function : receptor activity;

Not For Human Consumption!

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Customer Reviews (3)

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07/28/2023

    It has a high solubility and can be easily prepared at the desired concentration.

    07/26/2021

      It was found to be well active and could effectively perform the required biological activity experiments.

      04/15/2020

        It was observed that SLAMF7 had good crystallinity, which was conducive to crystallography research.

        Q&As (6)

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        What is the mechanism by which SLAMF7 protein regulates immune responses? 10/13/2022

        SLAMF7 protein is involved in regulating immune cell activation, proliferation, and transmission of background signals by binding to activating ligands, such as CD319, thereby influencing the immune response.

        What is the potential of combining SLAMF7 protein with immunotherapy? 05/02/2022

        By combining with immunotherapy, such as anti-PD-1 antibodies, the immune response can be further enhanced and the anti-tumor effect can be improved. The study of SLAMF7 protein provides a new direction for the development of combinatorial therapeutic strategies.

        In which tissues or cells is SLAMF7 protein expressed? 12/13/2021

        This protein is mainly expressed in the immune system, including immune cells such as plasma cells, NK cells, and activated T cells.

        Does the SLAMF7 protein have a similar role in other types of cancer? 11/14/2020

        The role of SLAMF7 protein in other types of cancer is unclear, and further research is needed to determine its potential role in other cancers.

        Does SLAMF7 protein have potential in the treatment of multiple myeloma? 05/20/2020

        Yes, the SLAMF7 protein has been shown to be a potential therapeutic target. A monoclonal antibody drug targeting SLAMF7 protein (Elotuzumab) has been developed for the treatment of multiple myeloma.

        What is the function of SLAMF7 protein in multiple myeloma? 12/11/2019

        SLAMF7 protein interacts with NK cells in multiple myeloma and plays a role in inhibiting and controlling tumor growth by activating the anti-tumor activity of NK cells.

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