Recombinant Human Slow Cardiac Myosin Regulatory light chain 2, His-tagged

• Cat.No.: MYL2-388H
• Product Overview: Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 20.9 kDa (186aa)

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD
• Formulation: Liquid in 20mM Tris-HCl (pH 8.0) containing 20% glycerol 5mM CaCl2
• Purity: > 95% by SDS PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: MYL2 myosin, light chain 2, regulatory, cardiac, slow [ Homo sapiens ]
• Synonyms: MYL2; myosin, light chain 2, regulatory, cardiac, slow; MLC2; CMH10; DKFZp779C0562; MYL2; slow cardiac myosin regulatory light chain 2; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2v; myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Gene ID: 4633
• mRNA Refseq: NM_000432
• Protein Refseq: NP_000423
• MIM: 160781
• UniProt ID: P10916
• Chromosome Location: 12q24.11
• Function: actin monomer binding; calcium ion binding; motor activity ;myosin heavy chain binding; protein binding; structural constituent of muscle