Recombinant Human SMAD1 Protein, MYC/DDK-tagged, C13 and N15-labeled

Cat.No. : SMAD1-333H
Product Overview : SMAD1 MS Standard C13 and N15-labeled recombinant protein (NP_001003688) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
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Description : The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 52.3 kDa
AA Sequence : MNVTSLFSFTSPAVKRLLGWKQGDE EEKWAEKAVDALVKKLKKKKGAMEE LEKALSCPGQPSNCVTIPRSLDGRL QVSHRKGLPHVIYCRVWRWPDLQSH HELKPLECCEFPFGSKQKEVCINPY HYKRVESPVLPPVLVPRHSEYNPQH SLLAQFRNLGQNEPHMPLNATFPDS FQQPNSHPFPHSPNSSYPNSPGSSS STYPHSPTSSDPGSPFQMPADTPPP AYLPPEDPMTQDGSQPMDTNMMAPP LPSEINRGDVQAVAYEEPKHWCSIV YYELNNRVGEAFHASSTSVLVDGFT DPSNNKNRFCLGLLSNVNRNSTIEN TRRHIGKGVHLYYVGGEVYAECLSD SSIFVQSRNCNYHHGFHPTTVCKIP SGCSLKIFNNQEFAQLLAQSVNHGF ETVYELTKMCTIRMSFVKGWGAEYH RQDVTSTPCWIEIHLHGPLQWLDKV LTQMGSPHNPISSVSTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE:
Gene Name : SMAD1 SMAD family member 1 [ Homo sapiens (human) ]
Official Symbol : SMAD1
Synonyms : SMAD1; SMAD family member 1; MAD, mothers against decapentaplegic homolog 1 (Drosophila), MADH1, SMAD, mothers against DPP homolog 1 (Drosophila); mothers against decapentaplegic homolog 1; JV4 1; MADR1; MAD homolog 1; Mad-related protein 1; TGF-beta signaling protein 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; MAD, mothers against decapentaplegic homolog 1; transforming growth factor-beta signaling protein 1; transforming growth factor-beta-signaling protein 1; BSP1; JV41; BSP-1; JV4-1; MADH1;
Gene ID : 4086
mRNA Refseq : NM_001003688
Protein Refseq : NP_001003688
MIM : 601595
UniProt ID : Q15797

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What technologies are commonly used to study the expression and activity of SMAD1 in clinical research? 04/12/2023

Techniques like Western blotting, immunohistochemistry, and qRT-PCR are commonly employed to study SMAD1 expression and activity in clinical research.

How might targeting SMAD1 have implications for anti-cancer therapies? 07/05/2022

Modulating SMAD1 activity could be explored as a potential strategy for developing anti-cancer therapies, particularly in cancers where the TGF-β pathway is dysregulated.

Are there any known genetic mutations in the SMAD1 gene associated with human diseases? 03/28/2021

Yes, mutations in the SMAD1 gene have been identified in certain individuals with congenital disorders and developmental abnormalities.

How does SMAD1 interact with other proteins in the TGF-β pathway? 01/08/2020

SMAD1 forms complexes with other SMAD proteins, transducing signals from TGF-β receptors to the nucleus, where they regulate gene expression.

How can SMAD1 be targeted for therapeutic interventions in bone-related disorders? 12/31/2019

Targeting SMAD1 or its downstream effectors in the TGF-β pathway may offer potential therapeutic strategies for bone-related disorders, such as osteoporosis.

Customer Reviews (3)

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06/08/2022

    Whether I am investigating enzymatic activity, studying protein-protein interactions, or testing its role in signaling pathways, the SMAD1 protein consistently performs exceptionally well.

    08/08/2021

      This versatility allows me to explore different aspects of my research with confidence and accuracy.

      05/02/2018

        The SMAD1 protein's excellent functionality makes it highly suitable for various trial applications.

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