Recombinant Human SMAD5 Protein, His-tagged

• Cat.No.: SMAD5-303H
• Product Overview: Recombinant Human SMAD5(Gly210~Val464) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 32.3kDa
• Protein length: Gly210~Val464
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: SMAD5 SMAD family member 5 [ Homo sapiens ]
• Official Symbol: SMAD5
• Synonyms: SMAD5; SMAD family member 5; MAD, mothers against decapentaplegic homolog 5 (Drosophila) , MADH5, SMAD, mothers against DPP homolog 5 (Drosophila); mothers against decapentaplegic homolog 5; Dwfc; JV5 1; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5; MAD, mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; DWFC; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323
• Gene ID: 4090
• mRNA Refseq: NM_001001419
• Protein Refseq: NP_001001419
• MIM: 603110
• UniProt ID: Q99717