Recombinant Human SMAD7 Protein, His-tagged

• Cat.No.: SMAD7-2705H
• Product Overview: Recombinant Human SMAD7 Protein (Trp261-Arg426) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23 kDa
  Accurate Molecular Mass: 23 kDa
• Protein length: Trp261-Arg426
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: SMAD7 SMAD family member 7 [ Homo sapiens (human) ]
• Official Symbol: SMAD7
• Synonyms: SMAD7; SMAD family member 7; MAD, mothers against decapentaplegic homolog 7 (Drosophila), MADH7, MADH8, SMAD, mothers against DPP homolog 7 (Drosophila); mothers against decapentaplegic homolog 7; hSMAD7; MAD homolog 8; mothers against DPP homolog 8; SMAD, mothers against DPP homolog 7; Mothers against decapentaplegic, drosophila, homolog of, 7; MAD (mothers against decapentaplegic, Drosophila) homolog 7; CRCS3; MADH7; MADH8; FLJ16482
• Gene ID: 4092
• mRNA Refseq: NM_001190821
• Protein Refseq: NP_001177750
• MIM: 602932
• UniProt ID: O15105