Recombinant Human SMARCA2 Protein (700-1216 aa), His-tagged

• Cat.No.: SMARCA2-1523H
• Product Overview: Recombinant Human SMARCA2 Protein (700-1216 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Neuroscience. Protein Description: Partial.

Specification

• Description: Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Belongs to the neural progenitors-specific chromatin rodeling complex (npBAF complex) and the neuron-specific chromatin rodeling complex (nBAF complex). During neural development a switch from a st/progenitor to a postmitotic chromatin rodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural st/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural st cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 61.7 kDa
• Protein length: 700-1216 aa
• AA Sequence: SYYTVAHAISERVEKQSALLINGTL KHYQLQGLEWMVSLYNNNLNGILAD EMGLGKTIQTIALITYLMEHKRLNG PYLIIVPLSTLSNWTYEFDKWAPSV VKISYKGTPAMRRSLVPQLRSGKFN VLLTTYEYIIKDKHILAKIRWKYMI VDEGHRMKNHHCKLTQVLNTHYVAP RRILLTGTPLQNKLPELWALLNFLL PTIFKSCSTFEQWFNAPFAMTGERV DLNEEETILIIRRLHKVLRPFLLRR LKKEVESQLPEKVEYVIKCDMSALQ KILYRHMQAKGILLTDGSEKDKKGK GGAKTLMNTIMQLRKICNHPYMFQH IEESFAEHLGYSNGVINGAELYRAS GKFELLDRILPKLRATNHRVLLFCQ MTSLMTIMEDYFAFRNFLYLRLDGT TKSEDRAALLKKFNEPGSQYFIFLL STRAGGLGLNLQAADTVVIFDSDWN PHQDLQAQDRAHRIGQQNEVRVLRL CTVNSVEEKILAAAKYKLNVDQKVI QAGMFDQKSSSHERRAF
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: SMARCA2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 [ Homo sapiens ]
• Official Symbol: SMARCA2
• Synonyms: SMARCA2; SNF2L2; BAF190; BRM; hBRM; hSNF2a; SNF2; SNF2LA; Sth1p; SWI2; BAF190B; SNF2-alpha; FLJ36757; MGC74511
• Gene ID: 6595
• mRNA Refseq: NM_003070
• Protein Refseq: NP_003061
• MIM: 600014
• UniProt ID: P51531