Recombinant Human SMC3 protein, His & T7-tagged

• Cat.No.: SMC3-1541H
• Product Overview: Recombinant Human SMC3 aa. (Ser994~Glu1181 (Accession # Q9UQE7)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 24.8kDa.
• Protein length: Ser994~Glu1181 (Accession # Q9UQE7)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: SMC3 structural maintenance of chromosomes 3 [ Homo sapiens (human) ]
• Official Symbol: SMC3
• Synonyms: BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1; SMC protein 3; basement membrane-associated chondroitin proteoglycan; chondroitin sulfate proteoglycan 6 (bamacan); chromosome-associated polypeptide; structural maintenance of chromosomes protein 3
• Gene ID: 9126
• mRNA Refseq: NM_005445.3
• Protein Refseq: NP_005436.1
• UniProt ID: Q9UQE7