Recombinant Human SMG8 protein, GST-tagged
Cat.No. : | SMG8-5743H |
Product Overview : | Recombinant Human SMG8 protein(521-745 aa), fused with N-terminal GST tag, was expressed in E.coli. |
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Species : | Human |
Source : | E.coli |
Tag : | GST |
Protein Length : | 521-745 aa |
Form : | The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 8.0). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 100 mM reduced glutathione (GSH). |
AASequence : | MTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNESSPAPPDSDADKLKEKEPQTQGESTSLSLALSLGQSTDSLGTYPADPQAGGDNPEVHGQVEVKTEKRPNFVDRQASTVE |
Purity : | 85%, by SDS-PAGE with Coomassie Brilliant Blue staining. |
Storage : | Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles. |
Reconstitution : | Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution. |
Gene Name | SMG8 smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) [ Homo sapiens ] |
Official Symbol | SMG8 |
Synonyms | SMG8; smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans); C17orf71, chromosome 17 open reading frame 71; protein SMG8; FLJ10587; FLJ23205; protein smg-8 homolog; amplified in breast cancer gene 2 protein; C17orf71; |
Gene ID | 55181 |
mRNA Refseq | NM_018149 |
Protein Refseq | NP_060619 |
MIM | 613175 |
UniProt ID | Q8ND04 |
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Not For Human Consumption!
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