Recombinant Human SMURF2 Protein (1-748 aa), His-tagged

• Cat.No.: SMURF2-1714H
• Product Overview: Recombinant Human SMURF2 Protein (1-748 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length.

Specification

• Description: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 88.2 kDa
• Protein length: 1-748 aa
• AA Sequence: MSNPGGRRNGPVKLRLTVLCAKNLV KKDFFRLPDPFAKVVVDGSGQCHST DTVKNTLDPKWNQHYDLYIGKSDSV TISVWNHKKIHKKQGAGFLGCVRLL SNAINRLKDTGYQRLDLCKLGPNDN DTVRGQIVVSLQSRDRIGTGGQVVD CSRLFDNDLPDGWEERRTASGRIQY LNHITRTTQWERPTRPASEYSSPGR PLSCFVDENTPISGTNGATCGQSSD PRLAERRVRSQRHRNYMSRTHLHTP PDLPEGYEQRTTQQGQVYFLHTQTG VSTWHDPRVPRDLSNINCEELGPLP PGWEIRNTATGRVYFVDHNNRTTQF TDPRLSANLHLVLNRQNQLKDQQQQ QVVSLCPDDTECLTVPRYKRDLVQK LKILRQELSQQQPQAGHCRIEVSRE EIFEESYRQVMKMRPKDLWKRLMIK FRGEEGLDYGGVAREWLYLLSHEML NPYYGLFQYSRDDIYTLQINPDSAV NPEHLSYFHFVGRIMGMAVFHGHYI DGGFTLPFYKQLLGKSITLDDMELV DPDLHNSLVWILENDITGVLDHTFC VEHNAYGEIIQHELKPNGKSIPVNE ENKKEYVRLYVNWRFLRGIEAQFLA LQKGFNEVIPQHLLKTFDEKELELI ICGLGKIDVNDWKVNTRLKHCTPDS NIVKWFWKAVEFFDEERRARLLQFV TGSSRVPLQGFKALQGAAGPRLFTI HQIDACTNNLPKAHTCFNRIDIPPY ESYEKLYEKLLTAIEETCGFAVE
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: SMURF2 SMAD specific E3 ubiquitin protein ligase 2 [ Homo sapiens ]
• Official Symbol: SMURF2
• Synonyms: SMURF2; hSMURF2; E3 ubiquitin ligase SMURF2; MGC138150; DKFZp686F0270
• Gene ID: 64750
• mRNA Refseq: NM_022739
• Protein Refseq: NP_073576
• MIM: 605532
• UniProt ID: Q9HAU4