Recombinant Human SNAP25 Protein, MYC/DDK-tagged, C13 and N15-labeled

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Recombinant Human SNAP25 Protein, MYC/DDK-tagged, C13 and N15-labeled

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Cat.No. : SNAP25-181H
Product Overview : SNAP25 MS Standard C13 and N15-labeled recombinant protein (NP_570824) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 23.1 kDa
AA Sequence : MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSGTRTR PLEQKLISEE DLAANDILDY KDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : SNAP25 synaptosomal-associated protein, 25kDa [ Homo sapiens (human) ]
Official Symbol : SNAP25
Synonyms : SNAP25; synaptosomal-associated protein, 25kDa; SNAP, synaptosomal associated protein, 25kD; synaptosomal-associated protein 25; bA416N4.2; dJ1068F16.2; resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SUP; super protein; SNAP; RIC-4; SNAP-25; FLJ23079;
Gene ID : 6616
mRNA Refseq : NM_130811
Protein Refseq : NP_570824
MIM : 600322
UniProt ID : P60880

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