Active Recombinant Full Length Human SNCA (A53T) Protein, Tag Free, low endotoxin

• Cat.No.: SNCA-77H
• Product Overview: Human Alpha-Synuclein (A53T), Tag Free is expressed from E.coli cells. It contains AA Met 1-Ala 140 (Accession # P37840-1 (A53T)). This protein carries no "tag". Predicted N-terminus: Met 1

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-140 aa
• Description: Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.
• Form: Supplied as 0.2 μm filtered solution in 50 mM HEPES, 100 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human Alpha-Synuclein (A53T), Tag Free at 1 μg/mL (100 μL/well) can bind Anti-Synucelin alpha/beta Monoclonal Antibody with a linear range of 0.1-4 ng/mL.
• Molecular Mass: The protein has a calculated MW of 14.5 kDa. The protein migrates as 15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 0.01 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at: The product MUST be stored at -70 centigrade or lower upon receipt; -70 centigrade for 3 months under sterile conditions. Please avoid repeated freeze-thaw cycles.
• Concentration: 0.2 mg/mL
• Shipping: This product is supplied and shipped with dry ice, please inquire the shipping cost.

Gene Information

• Gene Name: SNCA synuclein, alpha (non A4 component of amyloid precursor) [ Homo sapiens (human) ]
• Official Symbol: SNCA
• Synonyms: SNCA; synuclein, alpha (non A4 component of amyloid precursor); PARK1, PARK4, Parkinson disease (autosomal dominant, Lewy body) 4; alpha-synuclein; alpha synuclein; NACP; PD1; synuclein alpha-140; non A-beta component of AD amyloid; PARK1; PARK4; MGC110988
• Gene ID: 6622
• mRNA Refseq: NM_000345
• Protein Refseq: NP_000336
• MIM: 163890
• UniProt ID: P37840