Recombinant Human SNCA, A53T Mutant

• Cat.No.: SNCA-77H
• Product Overview: Recombinant Human A Parkinson's disease-related point mutant (A53T) of α-Synuclein. A53T Mutant of α-Synuclein was overexpressed inE.coliand the recombinant protein was purified to apparent homogeneity by taking advantage of the thermo solubility of the protein and by using conventional column chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Alpha-synuclein also known as SNCA is a protein which in humans is encoded by the SNCA gene. An alpha-synuclein fragment, known as the non-Abeta component (NAC) of Alzheimer"s disease amyloid, originally found in an amyloid-enriched fraction, is shown to be a fragment of its precursor protein, NACP, by cloning of the full-length cDNA. Later, NACP turned out to be a human homologue of Torpedo synuclein. Therefore, NACP is also referred to as human alpha-synuclein.
• Physical Appearance: Sterile filtered clorless solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.
• Formulation: The protein (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl .
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: SNCA synuclein, alpha (non A4 component of amyloid precursor) [ Homo sapiens ]
• Synonyms: SNCA; synuclein, alpha (non A4 component of amyloid precursor); PD1; NACP; PARK1; PARK4; MGC110988; Non-A beta component of AD amyloid;Alpha-synuclein;Non-A4 component of amyloid precursor; OTTHUMP00000161559; OTTHUMP00000161561; non A-beta component of AD amyloid
• Gene ID: 6622
• mRNA Refseq: NM_000345
• Protein Refseq: NP_000336
• UniProt ID: P37840
• Chromosome Location: 4q21.3-q22
• MIM: 163890
• Pathway: Alzheimer"s disease; Parkinson"s disease
• Function: Hsp70 protein binding; alpha-tubulin binding; achidonic acid binding; calcium ion binding; caspase inhibitor activity; dynein binding; fatty acid binding; fatty acid binding; ferrous iron binding; histone binding; identical protein binding; kinesin binding; magnesium ion binding; magnesium ion binding; phospholipase inhibitor activity; tau protein binding; transferrin transmembrane transporter activity; zinc ion binding