Recombinant Human SNCA protein, His-tagged SNCA-166H

Recombinant Human SNCA protein, His-tagged

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Recombinant Human SNCA protein, His-tagged

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Cat.No. : SNCA-166H
Product Overview : Recombinant Human TACSTD2 protein (Met1-Ala140) was fused to His-tag at the C-terminus and expressed in E.coli.
Description : Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
Source : E.coli
Species : Human
Tag : His
Predicted N Terminal : Met1
Form : Lyophilized from 0.22 μm filtered solution in 100 mM NaAC, pH7.0, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 16.3 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Met1-Ala140
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of SNCA-166H :

Human Alpha-Synuclein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Gene Name : SNCA
Official Symbol : SNCA
Synonyms : PD1; NACP; PARK1; PARK4
Gene ID : 6622
mRNA Refseq : NM_000345.4
Protein Refseq : NP_000336.1
MIM : 163890
UniProt ID : P37840

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