Active Recombinant Human SORD Protein

• Cat.No.: SORD-29H
• Product Overview: Recombinant human SORD protein, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: SORD, also known as sorbitol dehydrogenase, is a member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses.
• Source: E. coli
• Species: Human
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.5) containing 10% glycerol, 1mM DTT
• Bio-activity: Specific activity is >15 units/mg, and is defined as the amount of enzyme that catalyze the reduction 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 37C.
• Molecular Mass: 38.3kDa (357aa)
• AA Sequence: MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SORD sorbitol dehydrogenase [ Homo sapiens ]
• Official Symbol: SORD
• Synonyms: SORD; sorbitol dehydrogenase; L-iditol 2-dehydrogenase; SORD1
• Gene ID: 6652
• mRNA Refseq: NM_003104
• Protein Refseq: NP_003095
• MIM: 182500
• UniProt ID: Q00796
• Chromosome Location: 15q15-q21.1
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; sorbitol degradation I, organism-specific biosystem
• Function: L-iditol 2-dehydrogenase activity; L-iditol 2-dehydrogenase activity; NAD binding; metal ion binding; oxidoreductase activity; sugar binding; zinc ion binding; zinc ion binding