Recombinant Human SOS1 Protein, His-tagged

• Cat.No.: SOS1-13H
• Product Overview: The recombinant protein (amino acids 563-1049 of human SOS1) contains two domains, REM (Ras Exchanger Motif) and CDC25 (cell division cycle 25). CDC25 activates Ras and promotes nucleotide exchange on Ras proteins (2). The REM domain contains an activating allosteric site which binds Ras-GTP leading to additional stimulation of the CDC25 domain and potentiating the Ras-GTP exchange stimulated by SOS1.
  The human SOS1 (Lys563-Thr1049) fragment with a polyhistidine tag at the C-terminus was expressed in E. coli.

Specification

• Description: This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~58.3 kDa
• AA Sequence: MKEEQMRLPSADVYRFAEPDSEENI IFEENMQPKAGIPIIKAGTVIKLIE RLTYHMYADPNFVRTFLTTYRSFCK PQELLSLIIERFEIPEPEPTEADRI AIENGDQPLSAELKRFRKEYIQPVQ LRVLNVCRHWVEHHFYDFERDAYLL QRMEEFIGTVRGKAMKKWVESITKI IQRKKIARDNGPGHNITFQSSPPTV EWHISRPGHIETFDLLTLHPIEIAR QLTLLESDLYRAVQPSELVGSVWTK EDKEINSPNLLKMIRHTTNLTLWFE KCIVETENLEERVAVVSRIIEILQV FQELNNFNGVLEVVSAMNSSPVYRL DHTFEQIPSRQKKILEEAHELSEDH YKKYLAKLRSINPPCVPFFGIYLTN ILKTEEGNPEVLKRHGKELINFSKR RKVAEITGEIQQYQNQPYCLRVESD IKRFFENLNPMGNSMEKEFTDYLFN KSLEIEPRNPKPLPRFPKKYSYPLK SPGVRPSNPRPGTLEHHHHHH*
• Purity: > 90% by Coomassie staining
• Applications: Study inhibitors of SOS1 GTP exchange activity
  Identification of SOS1 binding proteins
  Study of SOS1 GEF activity with different GTPases
• Notes: This product is for laboratory research use only.
• Storage: Storage is recommended at -20 centigrade for longer periods of time.
• Concentration: 1.0-2.0 mg/mL by A280
• Storage Buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, 20% Glycerol.
• Shipping: Product requires shipping on ice packs.

Gene Information

• Gene Name: SOS1 SOS Ras/Rac guanine nucleotide exchange factor 1 [ Homo sapiens (human) ]
• Official Symbol: SOS1
• Synonyms: SOS1; SOS Ras/Rac guanine nucleotide exchange factor 1; GF1; HGF; NS4; GGF1; GINGF; SOS-1; son of sevenless homolog 1; gingival fibromatosis, hereditary, 1; guanine nucleotide exchange factor
• Gene ID: 6654
• mRNA Refseq: NM_005633
• Protein Refseq: NP_005624
• MIM: 182530
• UniProt ID: Q07889