Recombinant Human SPAG6 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human SPAG6 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : SPAG6-537H
Product Overview : SPAG6 MS Standard C13 and N15-labeled recombinant protein (NP_758442) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 49.6 kDa
AA Sequence : MSQRQVLQVF EQYQKARTQF VQMVAELATR PQNIETLQNA GVMSLLRTLL LDVVPTIQQI AALALGRLAN YNDDLAEAVV KCDILPQLVY SLAEQNRFYK KAAAFVLRAV GKHSPQLAQA IVDCGALDTL VICLEDFDPG VKEAAAWALR YIARHNAELS QAVVDAGAVP LLVLCIQEPE IALKGIAASA LSDIVKHSPE LAQTVVDAGA VAHLAQMILN PDAKLKHQIL SALSQVSKHS VDLAEMVVEA EIFPVVLTCL KDKDEYVKKN ASTLIREIAK HTPELSQLVV NAGGVAAVID CIGSCKGNTR LPGIMMLGYV AAHSENLAMA VIISKGVPQL SVCLSEEPED HIKAAAAWAL GQIGRHTPEH ARAVAVTNTL PVLLSLYMST ESSEDLQVKS KKAIKNILQK CTYLPALEPF LYDAPPNILK HVVGQFSKVL FPWIFRYTSA EGGQLSTTTR TRPLEQKLIS EEDLAANDIL DYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : SPAG6 sperm associated antigen 6 [ Homo sapiens (human) ]
Official Symbol : SPAG6
Synonyms : SPAG6; sperm associated antigen 6; sperm-associated antigen 6; axoneme central apparatus protein; pf16; Repro SA 1; protein PF16 homolog; sperm flagellar protein; Repro-SA-1; MGC26276; DKFZp434I153;
Gene ID : 9576
mRNA Refseq : NM_172242
Protein Refseq : NP_758442
MIM : 605730
UniProt ID : O75602

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