Recombinant Human SPP1 Protein, GST-tagged

• Cat.No.: SPP1-002H
• Product Overview: Recombinant Human SPP1 Protein (59-300 aa (partial length)) with GST tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: GST
• Molecular Mass: 55.8 kDa
• Protein length: 59-300
• Purity: >90% confirmed by 10% SDS-PAGE stained with Coomassie Blue (CB).
• Applications: ELISA, Western Blot, Antibody Production, Protein array
• Quality Control Test: 10% SDS-PAGE stained with Coomassie Blue (CB) immunoblotting with anti-GST (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry.
• Storage: Prior to reconstitution, store the lyophilized protein at 2−8 centigrade. After reconstitution, store in working aliquots at −80 centigrade.
• Expiry: All components are stable for 1 year of receipt, if stored properly.
• Storage Buffer: Lyophilized from a solution of phosphate buffered saline (PBS), pH 7.4, containing 0.50 mg SPP1 per ml. No preservatives added.
• Shipping: Lyophilized at Ambient temperature

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens (human) ]
• Official Symbol: SPP1
• Synonyms: SPP1; secreted phosphoprotein 1; OPN; BNSP; BSPI; ETA-1; osteopontin; PP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; urinary stone protein; uropontin
• Gene ID: 6696
• mRNA Refseq: NM_000582
• Protein Refseq: NP_000573
• MIM: 166490
• UniProt ID: P10451