Recombinant Human SPR Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SPR-5524H
• Product Overview: SPR MS Standard C13 and N15-labeled recombinant protein (NP_003115) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 28 kDa
• AA Sequence: MEGGLGRAVCLLTGASRGFGRTLAP LLASLLSPGSVLVLSARNDEALRQL EAELGAERSGLRVVRVPADLGAEAG LQQLLGALRELPRPKGLQRLLLINN AGSLGDVSKGFVDLSDSTQVNNYWA LNLTSMLCLTSSVLKAFPDSPGLNR TVVNISSLCALQPFKGWALYCAGKA ARDMLFQVLALEEPNVRVLNYAPGP LDTDMQQLARETSVDPDMRKGLQEL KAKGKLVDCKVSAQKLLSLLEKDEF KSGAHVDFYDKTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SPR sepiapterin reductase [ Homo sapiens (human) ]
• Official Symbol: SPR
• Synonyms: SPR; sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase); sepiapterin reductase; SDR38C1; short chain dehydrogenase/reductase family 38C; member 1; short chain dehydrogenase/reductase family 38C, member 1
• Gene ID: 6697
• mRNA Refseq: NM_003124
• Protein Refseq: NP_003115
• MIM: 182125
• UniProt ID: P35270