Recombinant Human STAT1 cell lysate

Cat.No. : STAT1-555HCL
Product Overview : Human STAT1 / p91 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : STAT1 signal transducer and activator of transcription 1, 91kDa [ Homo sapiens ]
Official Symbol : STAT1
Synonyms : STAT1; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1-alpha/beta; ISGF 3; STAT91; transcription factor ISGF 3 components p91/p84; transcription factor ISGF-3 components p91/p84; signal transducer and activator of transcription-1; CANDF7; ISGF-3; DKFZp686B04100;
Gene ID : 6772
mRNA Refseq : NM_007315
Protein Refseq : NP_009330
MIM : 600555
UniProt ID : P42224
Chromosome Location : 2q32.2-q32.3
Pathway : Adipogenesis, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;
Function : RNA polymerase II core promoter sequence-specific DNA binding; RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity; calcium ion binding; double-stranded DNA binding; enzyme binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; NOT sequence-specific DNA binding transcription factor activity; signal transducer activity; tumor necrosis factor receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the function of the STAT1 protein? 12/23/2019

STAT1 is a signal transduction and transcriptional activator involved in regulating a variety of biological processes such as cell growth, differentiation, apoptosis, and immune response.

Are mutations in genes associated with the STAT1 protein related to human disease? 09/21/2019

Mutations in the STAT1 gene have been found to be associated with a variety of diseases, including autoimmune diseases, primary immunodeficiency diseases, and malignant tumors.

What is the signaling pathway of STAT1 protein in immune cells? 09/01/2019

The STAT1 protein is primarily activated by the JAK-STAT signaling pathway. When activated by the receptor, JAK kinase phosphorylates the STAT1 protein, allowing it to form dimers and enter the nucleus to regulate transcription of related genes.

In which diseases does the STAT1 protein play an important role in the development of diseases? 07/16/2019

The STAT1 protein plays an important role in many diseases, including tumors, immune-related diseases (such as immunodeficiency diseases, autoimmune diseases), infectious diseases (such as viral infections), etc.

Are there activators or inhibitors of the STAT1 protein? 06/19/2019

Some activators or inhibitors of the STAT1 protein have been identified. These compounds can be used to study the function of the STAT1 protein and the development of disease treatments.

Is the STAT1 protein involved in the immune response to viral infection? 02/24/2019

The STAT1 protein plays an important role in viral infection. It mediates interferon signaling, promotes antiviral immune responses, and inhibits viral replication and transmission.

Customer Reviews (3)

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04/22/2020

    It is well suited for our laboratory to induce specific immune responses.

    11/26/2019

      It has great application potential and performs well in all aspects.

      04/16/2019

        This protein has high yield and high purity, which greatly improves our production efficiency.

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