Recombinant Human STAT3 Protein, His-tagged

• Cat.No.: STAT3-07H
• Product Overview: Recombinant human STAT3 protein (1-770aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 88.8 kDa
• Identity: 776aa
• AA Sequence: MAQWNQLQQLDTRYLEQLHQLYSDS FPMELRQFLAPWIESQDWAYAASKE SHATLVFHNLLGEIDQQYSRFLQES NVLYQHNLRRIKQFLQSRYLEKPME IARIVARCLWEESRLLQTAATAAQQ GGQANHPTAAVVTEKQQMLEQHLQD VRKRVQDLEQKMKVVENLQDDFDFN YKTLKSQGDMQDLNGNNQSVTRQKM QQLEQMLTALDQMRRSIVSELAGLL SAMEYVQKTLTDEELADWKRRQQIA CIGGPPNICLDRLENWITSLAESQL QTRQQIKKLEELQQKVSYKGDPIVQ HRPMLEERIVELFRNLMKSAFVVER QPCMPMHPDRPLVIKTGVQFTTKVR LLVKFPELNYQLKIKVCIDKDSGDV AALRGSRKFNILGTNTKVMNMEESN NGSLSAEFKHLTLREQRCGNGGRAN CDASLIVTEELHLITFETEVYHQGL KIDLETHSLPVVVISNICQMPNAWA SILWYNMLTNNPKNVNFFTKPPIGT WDQVAEVLSWQFSSTTKRGLSIEQL TTLAEKLLGPGVNYSGCQITWAKFC KENMAGKGFSFWVWLDNIIDLVKKY ILALWNEGYIMGFISKERERAILST KPPGTFLLRFSESSKEGGVTFTWVE KDISGKTQIQSVEPYTKQQLNNMSF AEIIMGYKIMDATNILVSPLVYLYP DIPKEEAFGKYCRPESQEHPEADPG SAAPYLKTKFCVTPTTCSNTIDLPM SPRTLDSLMQFGNNGEGAEPSAGGQ FESLTFDMELTSECATSPM
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Gene Information

• Gene Name: STAT3 signal transducer and activator of transcription 3 [ Homo sapiens (human) ]
• Official Symbol: STAT3
• Synonyms: STAT3; signal transducer and activator of transcription 3; APRF; HIES; ADMIO; ADMIO1; signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
• Gene ID: 6774
• mRNA Refseq: NM_139276
• Protein Refseq: NP_644805
• MIM: 102582
• UniProt ID: P40763

Reviews

11/17/2022

Protein worked well in assay.

04/21/2021

Fast shipping, great customer service.

02/06/2018

Excellent purity, highly recommend.

Q&As

Are there any known inhibitors targeting STAT3 for therapeutic purposes?

Yes, there are several inhibitors targeting STAT3, including Stattic, S3I-201, and OPB-31121, which are being explored for their therapeutic potential in cancer and other diseases.

Can STAT3 activation influence cell proliferation and survival?

Yes, activated STAT3 can promote cell proliferation and survival by inducing the expression of genes like cyclin D1 and Bcl-xL.

How does STAT3 contribute to oncogenesis and tumor progression?

STAT3 can contribute to oncogenesis by promoting cell proliferation, survival, angiogenesis, and immune evasion, and by suppressing apoptosis in various cancer types.

How can the activation of STAT3 be detected in cells?

Activation of STAT3 can be detected using Western blotting with phospho-specific antibodies that recognize the tyrosine-phosphorylated form of STAT3.

How does STAT3 interact with other signaling pathways in the cell?

STAT3 can interact with other signaling pathways such as the MAPK, PI3K/AKT, and NF-κB pathways, leading to integrated cellular responses.

What are the primary upstream activators of STAT3?

The primary upstream activators of STAT3 are cytokines and growth factors, especially those that signal through the Janus kinase (JAK) family, such as IL-6 and EGF.

What role does STAT3 play in immune cell differentiation and function?

STAT3 plays a crucial role in the differentiation of Th17 cells, a subset of CD4+ T cells, and is also involved in the function of myeloid-derived suppressor cells (MDSCs) and regulatory T cells, influencing immune responses.