Recombinant Human SULF1 Protein, His/GST-tagged

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Recombinant Human SULF1 Protein, His/GST-tagged

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Cat.No. : SULF1-667H
Product Overview : Recombinant Human SULF1(Pro609~Gly871) fused with His/GST tag at N-terminal was expressed in E. coli.
Description : This gene encodes an extracellular heparan sulfate endosulfatase. The encoded enzyme selectively removes 6-O-sulfate groups from heparan sulfate chains of heparan sulfate proteoglycans (HSPGs). The enzyme is secreted through the Golgi and is subsequently localized to the cell surface. The expression of this gene may be down-regulated in several types of cancer, including hepatocellular (HCC), ovarian and breast cancers. Alternative splicing results in multiple transcript variants.
Source : E. coli
Species : Human
Tag : His/GST
Form : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Molecular Mass : 61.08kDa
Protein length : Pro609~Gly871
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Figure :

Gene Name : SULF1 sulfatase 1 [ Homo sapiens ]
Official Symbol : SULF1
Synonyms : SULF1; sulfatase 1; extracellular sulfatase Sulf-1; KIAA1077; SULF 1; sulfatase FP; SULF-1; HSULF-1; FLJ30905; FLJ38022; FLJ41750;
Gene ID : 23213
mRNA Refseq : NM_001128204
Protein Refseq : NP_001121676
MIM : 610012
UniProt ID : Q8IWU6

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