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Recombinant Human TAS1R3 HEK293T cell lysate

Cat.No. : TAS1R3-18HCL
Product Overview : Human TAS1R3 over-expression lysate was derived from Human HEK293T cell line.
  • Specification
  • Gene Information
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Description : The protein encoded by this gene is a G-protein coupled receptor involved in taste responses. The encoded protein can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response.
Source : Human HEK293T cell line
Species : Human
Tag : C-terminal Myc/DDK
Form : The cell lysate is provided in RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4).
Molecular Mass : 93.3 kDa
Usage : 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage : The lysate is shipped with dry ice. Upon receiving, store the sample at -20centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20centigrade. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TAS1R3 taste receptor, type 1, member 3 [ Homo sapiens ]
Official Symbol : TAS1R3
Synonyms : TAS1R3; taste receptor, type 1, member 3; taste receptor type 1 member 3; T1R3; sweet taste receptor T1R3;
Gene ID : 83756
mRNA Refseq : NM_152228
Protein Refseq : NP_689414
MIM : 605865
UniProt ID : Q7RTX0
Chromosome Location : 1p36
Pathway : Carbohydrate digestion and absorption, organism-specific biosystem; Carbohydrate digestion and absorption, conserved biosystem; Class C/3 (Metabotropic glutamate/pheromone receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem; Taste transduction, organism-specific biosystem;
Function : protein heterodimerization activity; receptor activity; signal transducer activity; contributes_to sweet taste receptor activity; taste receptor activity; contributes_to taste receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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