Recombinant Human TCIRG1 Protein, His-tagged

• Cat.No.: TCIRG1-2753H
• Product Overview: Recombinant Human TCIRG1 Protein (Arg49-Val348) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 37.6 kDa
  Accurate Molecular Mass: 38 kDa
• Protein length: Arg49-Val348
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TCIRG1 T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 [ Homo sapiens (human) ]
• Official Symbol: TCIRG1
• Synonyms: TCIRG1; T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3; T cell, immune regulator 1, T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a isoform 3, T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3; V-type proton ATPase 116 kDa subunit a isoform 3; a3; Atp6i; ATP6N1C; ATP6V0A3; OC 116; OC116; T cell immune response cDNA 7; TIRC7; OC-116 kDa; V-ATPase 116-kDa; T-cell immune response cDNA 7; ATPase, H+ transporting, 116kD; V-type proton ATPase 116 kDa subunit a; osteoclastic proton pump 116 kDa subunit; specific 116-kDa vacuolar proton pump subunit; vacuolar proton translocating ATPase 116 kDa subunit A; T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a; Stv1; Vph1; OPTB1; OC-116kDa
• Gene ID: 10312
• mRNA Refseq: NM_006019
• Protein Refseq: NP_006010
• MIM: 604592
• UniProt ID: Q13488