Recombinant Human Tec Protein Tyrosine Kinase, GST-tagged

• Cat.No.: TEC-1111H
• Product Overview: Full-length recombinant human TEC was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: TEC is a member of the Tec family of non-receptor protein-tyrosine kinases that are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. TEC is an integral component of T cell signaling and has a distinct role in T cell activation. Defects in TEC may be associated with myelodysplastic syndrome. TEC plays a crucial role in regulating FGF2 secretion under various physiological conditions and it inhibits CD25 expression in human T-lymphocyte.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 103 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Specific Activity: 10 nmol/min/mg
• Purity: > 75 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: T cell receptor signaling pathway

Gene Information

• Gene Name: TEC tec protein tyrosine kinase [ Homo sapiens ]
• Official Symbol: TEC
• Synonyms: TEC; tec protein tyrosine kinase; PSCTK4; MGC126760; MGC126762; tyrosine-protein kinase Tec; EC 2.7.10.2
• Gene ID: 7006
• mRNA Refseq: NM_003215
• Protein Refseq: NP_003206
• MIM: 600583
• UniProt ID: P42680
• Chromosome Location: 4p12
• Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; transferase activity