"TFRC" Related Products

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Recombinant Human TFRC Protein, His-tagged, Biotinylated

Cat.No.: TFRC-3865H
Product Overview: Biotinylated Human Transferrin R / CD71 is expressed from HEK293 cells. It contains AA Cys 89 - Phe 760 (Accession # AAH01188.1). This protein carries a polyhistidine tag at the N-terminus.
Source: HEK293
Species: Human
Tag : His
Predicted N Terminal: His
Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity: Immobilized Human Transferrin, His Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human Transferrin R, His Tag with a linear range of 5-156 ug/mL.
Molecular Mass: The protein has a calculated MW of 76 kDa. The protein migrates as 80-85 kDa on a SDS-PAGE gel under reducing condition due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >92% as determined by SDS-PAGE.
Characteristic: The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product. The biotin to protein ratio is 1-2 as determined by the HABA assay.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: TFRC transferrin receptor (p90, CD71) [ Homo sapiens ]
Official Symbol: TFRC
Synonyms: TFRC; transferrin receptor (p90, CD71); transferrin receptor protein 1; CD71; TFR1; T9; TR; TFR; p90; TRFR;
Gene ID: 7037
mRNA Refseq: NM_001128148
Protein Refseq: NP_001121620
MIM: 190010
UniProt ID: P02786

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