Active Recombinant Human TFRC Protein, His-tagged, Biotinylated

• Cat.No.: TFRC-3865H
• Product Overview: Biotinylated Human Transferrin R / CD71 is expressed from HEK293 cells. It contains AA Cys 89 - Phe 760 (Accession # AAH01188.1). This protein carries a polyhistidine tag at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human Transferrin, His Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human Transferrin R, His Tag with a linear range of 5-156 ug/mL.
• Molecular Mass: The protein has a calculated MW of 76 kDa. The protein migrates as 80-85 kDa on a SDS-PAGE gel under reducing condition due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Characteristic: The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product. The biotin to protein ratio is 1-2 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TFRC transferrin receptor (p90, CD71) [ Homo sapiens ]
• Official Symbol: TFRC
• Synonyms: TFRC; transferrin receptor (p90, CD71); transferrin receptor protein 1; CD71; TFR1; T9; TR; TFR; p90; TRFR
• Gene ID: 7037
• mRNA Refseq: NM_001128148
• Protein Refseq: NP_001121620
• MIM: 190010
• UniProt ID: P02786