Recombinant Human TGFA protein, hFc-tagged

• Cat.No.: TGFA-6744H
• Product Overview: Recombinant Human TGFA protein(P01135-1)(Val40-Ala89), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Val40-Ala89
• Tag: N-hFc
• Form: Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TGFA transforming growth factor, alpha [ Homo sapiens ]
• Official Symbol: TGFA
• Synonyms: TGFA; transforming growth factor, alpha; protransforming growth factor alpha; TGF-alpha; TFGA
• Gene ID: 7039
• mRNA Refseq: NM_001099691
• Protein Refseq: NP_001093161
• MIM: 190170
• UniProt ID: P01135