Active Recombinant Human TGFB1 Protein, Fc tagged

• Cat.No.: TGFB1-706H
• Product Overview: The mutant human TGF-β1 (including aa 30-278 (Latency-Associated Peptide, LAP) and aa279-390 (TGF-β1)) is fused to the N-terminus of the Fc region of human IgG4. Site-directed mutagenesis was used to change three cysteine codons into a serine codon that are located in the pro region of the TGF-β precursor at amino acid positions 33, 223, and 225.

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
• Form: Lyophilized from 0.2μm filtered solution in PBS.
• Bio-activity: Shows the biological function of the TGFβ1 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
• Endotoxin: < 0.06 EU/μg protein
• Purity: ≥ 98% (SDS-PAGE)
• Storage: Short Term Storage: +4 centigrade Long Term Storage: -20 centigrade Use/Stability: Stable for at least 1 year after receipt when stored at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
• Shipping: BLUE ICE
• Reconstitution: Reconstitute with 100 μl sterile water. Add 1× PBS to the desired protein concentration.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens (human) ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137