Recombinant Human TIGAR Protein, His-tagged

• Cat.No.: TIGAR-404H
• Product Overview: Recombinant Human TIGAR Protein (Met1-Arg270) with N-His tag was expressed in E. coli.

Specification

• Description: This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.8 kDa
  Accurate Molecular Mass: 36 kDa
• Protein length: Met1-Arg270
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TIGAR TP53 induced glycolysis regulatory phosphatase [ Homo sapiens (human) ]
• Official Symbol: TIGAR
• Synonyms: TIGAR; TP53 induced glycolysis regulatory phosphatase; FR2BP; C12orf5; fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; transactivated by NS3TP2 protein; EC 3.1.3.46
• Gene ID: 57103
• mRNA Refseq: NM_020375
• Protein Refseq: NP_065108
• MIM: 610775
• UniProt ID: Q9NQ88