Active Recombinant Human TIMP3 Protein, Tag Free

• Cat.No.: TIMP3-2528H
• Product Overview: Recombinant Human TIMP-3 Protein (Cys24-Pro211) without tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: NS0
• Tag: Non
• Protein Length: 24-211 aa
• Description: This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.
• Form: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The IC50 value is approximately 3 nM, under conditions the described conditions.
• Molecular Mass: 22 kDa
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Shipping: The product is shipped with polar packs.
• Reconstitution: Reconstitute at 100 μg/mL in sterile, deionized water.
• N-terminal Sequence Analysis: Cys24

Gene Information

• Gene Name: TIMP3 TIMP metallopeptidase inhibitor 3 [ Homo sapiens ]
• Official Symbol: TIMP3
• Synonyms: TIMP3; TIMP metallopeptidase inhibitor 3; SFD, tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory); metalloproteinase inhibitor 3; TIMP-3; MIG-5 protein; protein MIG-5; tissue inhibitor of metalloproteinases 3; SFD; K222; K222TA2; HSMRK222
• Gene ID: 7078
• mRNA Refseq: NM_000362
• Protein Refseq: NP_000353
• MIM: 188826
• UniProt ID: P35625