Recombinant Human TNF Protein, His-tagged

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Recombinant Human TNF Protein, His-tagged

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Cat.No. : TNF-2001H
Product Overview : Recombinant Human TNF-alpha Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val77-Leu233) of human TNF-alpha (Accession #NP_000585.2) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.
Description : This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Source : E. coli
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol.
Protein length : Val77-Leu233
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : > 90% as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : tumor necrosis factor [ Homo sapiens (human) ]
Official Symbol : TNF
Synonyms : DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F
Gene ID : 7124
mRNA Refseq : NM_000594.3
Protein Refseq : NP_000585.2
MIM : 191160
UniProt ID : P01375
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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