Active Recombinant Human TNFRSF9 protein(Met1-Gln186), hFc-tagged

• Cat.No.: TNFRSF9-43H
• Product Overview: Recombinant Human TNFRSF9 protein(NP_001552.2)(Met1-Gln186), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Gln186
• similar: TNFRSF9
• Alias: TNFRSF9; tumor necrosis factor receptor superfamily, member 9; ILA; tumor necrosis factor receptor superfamily member 9; 4 1BB; CD137; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; 4-1BB; CDw137; MGC2172; FLJ43501
• Conjugate: C-hFc
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bioactivity: Measured by its binding ability in a functional ELISA. Immobilized TNFRSF9-Fc at 10 μg/ml(100 μl/well) can bind TNFSF9/Biotin, The EC50 of TNFSF9/Biotin is 20-60 ng/mL.
• MolecularMass: The recombinant human TNFRSF9 consists of 401 amino acids and predicts a molecular mass of 44 kDa. As a result of glycosylation, it migrates as an approximately 58.9 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• GeneName: TNFRSF9 tumor necrosis factor receptor superfamily, member 9 [ Homo sapiens ]
• GeneNameWeblink: 3604
• OfficialSymbol: TNFRSF9
• OfficialSymbolWeblink: 3604
• GeneID: 3604
• GeneIDWeblink: 3604
• mRNARefseq: NM_001561
• mRNARefseqWeblink: NM_001561.5
• ProteinRefseqWeblink: NP_001552.2
• MIMWeblink: 602250
• UniProtID: Q07011
• UniProtIDWeblink: Q07011
• Publications:
  • Epitope and Fc-Mediated Cross-linking, but Not High Affinity, Are Critical for Antitumor Activity of CD137 Agonist Antibody with Reduced Liver Toxicity (2020)

Gene Information

• Synonyms: TNFRSF9; tumor necrosis factor receptor superfamily, member 9; ILA; tumor necrosis factor receptor superfamily member 9; 4 1BB; CD137; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; 4-1BB; CDw137; MGC2172; FLJ43501
• MIM: 602250