Species : |
Human |
Source : |
E.coli |
Tag : |
Non |
Description : |
The TNF-related ligand, B cell-activating factor belonging to the TNF family (BAFF), is necessary for normal B cell development and survival, and specifically binds the receptors transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI), B cell maturation Ag (BCMA), and BAFF-R.Using specific mAbs, BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice, and antagonist mAbs to BAFF-R blocked BAFF-mediated costimulation of anti- micro responses. The other BAFF receptors showed a much more restricted expression pattern, suggestive of specialized roles. |
Amino Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Val-Gln-Gly. |
Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation : |
Each mg contains 1.6mg mannitol and 1.2mg HSA. |
Solubility : |
It is recommended to reconstitute the lyophilized BAFF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability : |
Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BAFF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Purity : |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
Dimers And Aggregates : |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
Biological Activity : |
Feldan Bio's recombinant B Lymphocyte Stimulator is fully biologically active when compared to standard. The activity is determined by the dose-dependant stimulation of IL-8 production by human PBMC. The ED50 for this effect is less than 10ng/ml. Please note: results may vary with PBMC donors. |
Endotoxin : |
Less than 0.1 ng/µg (IEU/µg) of BAFF. |
Protein Content : |
Protein quantitation was carried out by two independent methods:1.UV spectroscopy at 280. 2. Analysis by RP-HPLC, using a standard solution of BAFF as a Reference Standard. |