Recombinant Human TNFSF13B Protein, Ala134-Leu285, N-10×His tagged

• Cat.No.: TNFSF13B-11H
• Product Overview: Recombinant Human TNFSF13B Protein (Ala134-Leu285) with N-10×His was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: Mouse myeloma cell
• Tag: His
• Protein Length: Ala134-Leu285
• Description: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
• Bio-activity: Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.4-2 ng/mL in the presence of goat anti-mouse IgM μ chain.
• Molecular Mass: Predicted Molecular Mass: 19.6 kDa
  SDS-PAGE: 22-29 kDa, reducing conditions
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: Met
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• References: 1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
  2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
  3. Moore, P.A. et al. (1999) Science 285:260.

Gene Information

• Gene Name: TNFSF13B TNF superfamily member 13b [ Homo sapiens (human) ]
• Official Symbol: TNFSF13B
• Synonyms: TNFSF13B; TNF superfamily member 13b; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20; tumor necrosis factor ligand superfamily member 13B; ApoL related ligand TALL-1; B-cell-activating factor; B-lymphocyte stimulator; Delta4 BAFF; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; delta BAFF; dendritic cell-derived TNF-like molecule; epididymis secretory sperm binding protein; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; tumor necrosis factor superfamily member 13b; tumor necrosis factor-like protein ZTNF4
• Gene ID: 10673
• mRNA Refseq: NM_006573
• Protein Refseq: NP_006564
• MIM: 603969
• UniProt ID: Q9Y275