Recombinant Human TNFSF13B protein, His-tagged

Cat.No.: TNFSF13B-1818H
Product Overview: Human BAFF, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 134 - Leu 285 (Accession # AAH20674.1).
Description: B-cell activating factor (BAFF) is also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.
Source: HEK293
Species: Human
Tag : His
Predicted N Terminal: His
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity: Immobilized Human BAFF, His Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc Tag with a linear range of 3.9-15.6 ng/mL.
Molecular Mass: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 18.4 kDa. The protein migrates as 20 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Protein length: AA Ala 134 - Leu 285
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: TNFSF13B TNF superfamily member 13b [ Homo sapiens (human) ]
Official Symbol: TNFSF13B
Synonyms: DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20; ApoL related ligand TALL-1; B-cell-activating factor; B-lymphocyte stimulator; Delta4 BAFF; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; delta BAFF; dendritic cell-derived TNF-like molecule; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; tumor necrosis factor superfamily member 13b; tumor necrosis factor-like protein ZTNF4
Gene ID: 10673
mRNA Refseq: NM_001145645.2
Protein Refseq: NP_001139117.1
UniProt ID: Q9Y275

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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