Active Recombinant Human TNFSF15 protein, His-Flag-tagged, Low Endotoxin

• Cat.No.: TNFSF15-150H
• Product Overview: Recombinant Human TNFSF15 protein(O95150-1)(91-251 aa), fused with N-terminal His and Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 91-251 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TNFSF15 Trimer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 2.2μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFSF15 tumor necrosis factor (ligand) superfamily, member 15 [ Homo sapiens ]
• Official Symbol: TNFSF15
• Synonyms: TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; MGC129934; MGC129935; TL1; TL1A; TNF ligand related molecule 1; TNF superfamily ligand TL1A; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor 192A; VEGI; VEGI192A; TNF ligand-related molecule 1; vascular endothelial growth inhibitor-192A
• Gene ID: 9966
• mRNA Refseq: NM_001204344
• Protein Refseq: NP_001191273
• MIM: 604052
• UniProt ID: O95150