Recombinant Human TNFSF18 Protein, Fc-Avi-tagged, Biotinylated

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Recombinant Human TNFSF18 Protein, Fc-Avi-tagged, Biotinylated

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Cat.No. : TNFSF18-38H
Product Overview : Biotinylated Human GITR Ligand, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1). This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc tag.
Source : HEK293
Species : Human
Tag : Fc-Avi
Predicted N Terminal : Gly
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity : Immobilized Human GITR, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human GITR Ligand, Fc Tag, Avi Tag with a linear range of 3.9-62.5 ug/mL.
Molecular Mass : The protein has a calculated MW of 43.5 kDa. The protein migrates as 45-53 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : TNFSF18 tumor necrosis factor (ligand) superfamily, member 18 [ Homo sapiens ]
Official Symbol : TNFSF18
Synonyms : TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18; AITRL; hGITRL; TL6; AITR ligand; GITR ligand; activation-inducible TNF-related ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; GITRL; MGC138237;
Gene ID : 8995
mRNA Refseq : NM_005092
Protein Refseq : NP_005083
MIM : 603898
UniProt ID : Q9UNG2

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