Recombinant Human TNFSF18 protein, His-tagged
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|Product Overview :||Recombinant Human TNFSF18 (Gln50-Ser177) protein was fused to His-tag at N-terminus and expressed in human 293 cells (HEK293).|
|Description :||Tumor necrosis factor ligand superfamily member 18 (TNFSF18) is also known as Glucocorticoid - induced TNF-related ligand (GITRL or GITR Ligand), Activation - inducible TNF - related ligand (AITRL), which belongs to the tumor necrosis factor family. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. TNFSF18/GITRL is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18/GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells. TNFSF18 also mediates activation of NF-kappa-B.|
|Predicted N Terminal :||Gln50|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 20.5 kDa. The protein migrates as 23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Gln50-Ser177|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of TNFSF18-722H :||
Human GITR Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of TNFSF18-722H :||
Immobilized Human GITR Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Human GITR, Fc Tag with a linear range of 0.1-4 ng/mL (QC tested).
Immobilized Human GITR Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Human GITR, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.2-8 ng/mL (Routinely tested).
|Gene Name :||TNFSF18|
|Official Symbol :||TNFSF18|
|Synonyms :||TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18; AITRL; hGITRL; TL6; AITR ligand; GITR ligand; activation-inducible TNF-related ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; GITRL; MGC138237|
|Gene ID :||8995|
|mRNA Refseq :||NM_005092|
|Protein Refseq :||NP_005083|
|UniProt ID :||Q9UNG2|
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