Recombinant Human TNK2 Protein, His-tagged

• Cat.No.: TNK2-458H
• Product Overview: Recombinant Human TNK2 Protein (Leu126-Leu385) with N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Leu126-Leu385
• Description: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.5 kDa
  Accurate Molecular Mass: 33 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TNK2 tyrosine kinase, non-receptor, 2 [ Homo sapiens (human) ]
• Official Symbol: TNK2
• Synonyms: TNK2; tyrosine kinase, non-receptor, 2; activated CDC42 kinase 1; ACK; ACK1; activated Cdc42 associated kinase 1; p21cdc42Hs; activated p21cdc42Hs kinase; activated Cdc42-associated kinase 1; tyrosine kinase non-receptor protein 2; ACK-1; FLJ44758; FLJ45547
• Gene ID: 10188
• mRNA Refseq: NM_001010938
• Protein Refseq: NP_001010938
• MIM: 606994
• UniProt ID: Q07912