Recombinant Human TOPBP1 Protein, His tagged

• Cat.No.: TOPBP1-32H
• Product Overview: Purified recombinant protein of Human topoisomerase (DNA) II binding protein 1 (TOPBP1), Lys892-Asp1146, with N-terminal His tag, expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 892-1146 aa
• Description: This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
• Molecular Weight: 28.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Storage Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea
• Reconstitution: >0.05 μg/μL as determined by microplate BCA method

Gene Information

• Gene Name: TOPBP1 DNA topoisomerase II binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: TOPBP1
• Synonyms: TOPBP1; topoisomerase (DNA) II binding protein 1; DNA topoisomerase 2-binding protein 1; KIAA0259; TOP2BP1; DNA topoisomerase II-binding protein 1; DNA topoisomerase II-beta-binding protein 1
• Gene ID: 11073
• mRNA Refseq: NM_007027
• Protein Refseq: NP_008958
• MIM: 607760
• UniProt ID: Q92547