Recombinant Human TP53RK, GST-tagged

• Cat.No.: TP53RK-197H
• Product Overview: Recombinant full-length human TP53RK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: TP53RK or TP53 regulating kinase and also called PRPK (p53-related protein kinase) is the human homologue of yeast Bud32. TP53RK belongs to a small subfamily of atypical protein kinases. TP53RK is expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes and lower expression of TP53RK is found in heart, kidney, and spleen. PRPK interacts with CGI-121 and co-precipitation of p53 with PRPK is inhibited by adding recombinant CGI-121 in vitro. CGI-121 may act as an inhibitor of the PRPK-p53 interaction. TP53RK is activated by another kinase, Akt/PKB, which phosphorylates TP53RK at Ser250.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~55 kDa
• Purity: >80% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: TP53RK TP53 regulating kinase [ Homo sapiens ]
• Official Symbol: TP53RK
• Synonyms: TP53RK; TP53 regulating kinase; C20orf64, chromosome 20 open reading frame 64; TP53-regulating kinase; BUD32; dJ101A2.2; Nori 2p; prpk; p53-related protein kinase; PRPK; Nori-2; Nori-2p; dJ101A2; C20orf64
• Gene ID: 112858
• mRNA Refseq: NM_033550
• Protein Refseq: NP_291028
• MIM: 608679
• UniProt ID: Q96S44
• Chromosome Location: 20q13.2
• Function: ATP binding; nucleotide binding; p53 binding; protein binding; protein serine/threonine kinase activity; protein tyrosine kinase activity