Active Recombinant Human TPBG protein, His-Avi-tagged, Biotinylated

• Cat.No.: TPBG-33H
• Product Overview: Biotinylated Recombinant Human TPBG protein(Ser 32 - Ser 355), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Biotinylated Human TPBG, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-TPBG Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Ser 32 - Ser 355
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TPBG trophoblast glycoprotein [ Homo sapiens ]
• Official Symbol: TPBG
• Synonyms: TPBG; trophoblast glycoprotein; 5T4; 5T4 AG; 5T4 oncofetal antigen; 5T4 oncotrophoblast glycoprotein; 5T4 oncofetal trophoblast glycoprotein; M6P1; 5T4AG
• Gene ID: 7162
• mRNA Refseq: NM_001166392
• Protein Refseq: NP_001159864
• MIM: 190920
• UniProt ID: Q13641